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Journal of Lasers in Medical Sciences 2023Despite the advanced formulations of resin composites, microleakage is still among the commonest causes of clinical failure of these restorations. We evaluated the...
Despite the advanced formulations of resin composites, microleakage is still among the commonest causes of clinical failure of these restorations. We evaluated the effect of surface coating and laser pretreatment on the microleakage of Class V resin composite restorations in primary teeth. Sixty extracted primary molar teeth having intact lingual or facial surfaces were randomly allocated into the control, G-Coat Plus surface coating, and erbium-doped yttrium aluminum garnet (Er:YAG) laser pretreatment groups. Class V cavities were provided with the coronal and gingival margins in the dentin and enamel, respectively. Restoration of the cavities was done with Z250 resin composite and they were thermocycled, followed by immersing in 2% basic Fuchsin dye for 24 hrs. Samples underwent sectioning occlusogingivally and the microleakage was assessed under a stereomicroscope (40×). Statistical analysis was done via SPSS and Kruskal-Wallis test (α=0.05). The control and G-Coat plus groups were significantly different regarding the microleakage (<0.001), and G-Coat Plus and laser pretreatment groups (<0.001) at both gingival and occlusal margins. However, it showed no significant difference between the laser and the control group on the enamel (=0.063) and dentin margins (=0.757). Microleakage at the gingival margins was significantly greater compared to the occlusal margins in the control and laser groups (<0.001), but not in the G-Coat Plus group (=0.051). G-Coat plus coating significantly reduced microleakage at dentin and enamel margins of Class V composite restorations in primary teeth, in comparison with other groups of the study. Also, dentin margins showed more significant amounts of microleakage versus enamel margins in all groups, except for the G-Coat Plus group.
PubMed: 37583502
DOI: 10.34172/jlms.2023.11 -
RSC Advances Nov 2018Cyclodextrin polymers are efficient adsorbents for dye adsorption. Herein, a β-cyclodextrin polymer (β-CDSP) with carboxyl groups and benzene rings was prepared free...
Cyclodextrin polymers are efficient adsorbents for dye adsorption. Herein, a β-cyclodextrin polymer (β-CDSP) with carboxyl groups and benzene rings was prepared free radical polymerization of β-cyclodextrin-maleate and styrene. The adsorption performance of β-CDSP was studied by adsorbing neutral red (NR), basic fuchsin (BF) and safranine T (ST) dyes under different adsorption conditions (, adsorption time, temperature and pH of the solution). The results showed that the adsorption of BF and ST was faster and better than that of NR. The adsorption kinetic behavior fitted well with both the pseudo-first-order and the pseudo-second-order models for NR and BF, but it fitted better with the latter for ST. The adsorption equilibrium data followed the Langmuir isotherm model. The adsorption process was endothermic and spontaneous, and a higher temperature was favorable for dye adsorption. Higher values were obtained in a basic medium, which resulted from the electrostatic interactions between β-CDSP and cationic dyes. Furthermore, inclusion complexion and π-π interactions also contributed to the dye adsorption. The stability and reusability of β-CDSP were estimated by four regeneration cycles.
PubMed: 35558233
DOI: 10.1039/c8ra07709f -
Auris, Nasus, Larynx Feb 2023We aim to identify the potential genes and signaling pathways associated with the nasopharyngeal carcinoma (NPC) prognosis using Weighted Gene Co-Expression Network...
OBJECTIVE
We aim to identify the potential genes and signaling pathways associated with the nasopharyngeal carcinoma (NPC) prognosis using Weighted Gene Co-Expression Network Analysis (WGCNA).
METHODS
Gene Expression Omnibus (GEO) query was utilized to download two NPC mRNA microarray data. WGCNA was conducted on differentially expressed genes (DEGs) to obtain tumor-associated gene modules. Genes in core modules were intersected with DEGs for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis. GSE102349 dataset was devoted to identifying prognostic hub genes by survival analysis and the results were confirmed by quantitative polymerase chain reaction (qPCR).
RESULTS
Co-expression networks were built, and we detected 12 gene modules. The Brown module and Magenta module were extremely associated with NPC samples. GO functional analysis and KEGG pathway analysis was carried out to the genes in the Brown and Magenta modules. Our data indicated that DEGs in Brown module and Magenta module were correlated with the biological regulation, metabolic process, reproduction, and cellular proliferation. Twenty-six hub genes were obtained and were considered to be closely related to NPC. GSE102349 dataset was devoted to identifying prognostic hub genes by survival analysis. The expression of IL33, MPP3 and SLC16A7 in GSE102349 dataset was significantly correlated with the progression-free survival (PFS). The results of qPCR indicated a strong correlation between SLC16A7 expression and the overall survival (OS).
CONCLUSIONS
WGCNA contributed to the detection of gene modules and identification of hub genes and crucial genes. These crucial genes might be potential targets for pharmaceutic therapies with potential clinical significance.
Topics: Humans; Nasopharyngeal Carcinoma; Rosaniline Dyes; Gene Expression Profiling; Nasopharyngeal Neoplasms
PubMed: 35659152
DOI: 10.1016/j.anl.2022.05.013 -
Global Challenges (Hoboken, NJ) Nov 2022Adsorption and advanced oxidation processes, especially photocatalysis, are amongst the most common water treatment methodologies. Unfortunately, using each of these...
Adsorption and advanced oxidation processes, especially photocatalysis, are amongst the most common water treatment methodologies. Unfortunately, using each of these techniques independently does not fully eliminate the pollutants of diverse nature, which are present in wastewater. Here, an avenue for multifunctional materials for water treatment is opened by reporting for the first time the preparation, characterization, and study of the properties of a novel multifunctional nanocomposite with both adsorption and visible-light-driven photocatalysis abilities. These multifunctional nanocomposites, namely iron (II, III) oxide/poly(-isopropylacrylamide--methacrylic acid)/silver-titanium dioxide (FeO/P(NIPAM--MAA)/Ag-TiO), are prepared by combining magnetic polymeric microspheres (FeO/P(NIPAM--MAA)) with silver-decorated titanium dioxide nanoparticles (Ag-TiO NPs). Cationic dyes, such as basic fuchsin (BF), can be adsorbed by the nanocomposites thanks to the carboxylic groups of FeO/P(NIPAM--MAA) microspheres. Concomitantly, the presence of Ag-TiO NPs endows the system with the visible-light-driven photocatalytic degradation ability toward antibiotics such as ciprofloxacin (CIP) and norfloxacin (NFX). Furthermore, the proposed nanocomposites show antibacterial activity toward (), thanks to the presence of silver nanoparticles (Ag NPs). Due to the superparamagnetic properties of iron (II, III) oxide nanoparticles (FeO NPs), the nanocomposites can be also recycled and reused, after the cleaning process, by using an external magnetic field.
PubMed: 36381130
DOI: 10.1002/gch2.202200076 -
RSC Advances Aug 2022To provide a low-cost photocatalyst and new methodology for the utilization of waste rice noodle (WRN), a carbon quantum dots/zinc oxide (CQDs/ZnO) composite using WRN...
To provide a low-cost photocatalyst and new methodology for the utilization of waste rice noodle (WRN), a carbon quantum dots/zinc oxide (CQDs/ZnO) composite using WRN as the raw material was synthesized and characterized. The CQDs/ZnO composite based on WRN exhibited a highly efficient photocatalytic degradation effect on various organic pollutants and could be a good alternative for commercial ZnO. For methylene blue, the CQDs/ZnO composite showed a good degradation rate of 99.58% within 40 min, a high degradation rate constant of 0.2630 min, and could be recycled and reused for ten photocatalytic cycles without an appreciable decrease in the degradation effect, which was much better than that of commercial ZnO. The resulting CQDs/ZnO composite also displayed a nice photocatalytic degradation effect on other common organic pollutants, such as malachite green, methyl violet, basic fuchsin, rhodamine B, aniline and tetracycline. In particular, it could achieve excellent photocatalytic degradation on malachite green with an extremely high degradation rate constant of 1.9260 min. Besides, the CQDs/ZnO composite could also be used to control the pollution of tetracycline or aniline. The introduction of CQDs based on WRN to ZnO resulted in efficient electron-hole pair separation and enabled more photogenerated electrons to reduce O and more photogenerated holes to oxidize HO, which caused stronger abilities in producing radicals (such as O˙ and ˙OH) and a better photocatalytic degradation effect to organic pollutants.
PubMed: 36090407
DOI: 10.1039/d2ra03709b -
Veterinary World Nov 2018The purpose of this work was to study the dynamics of structural manifestations of acute cases of postpartum endometritis in cows.
AIM
The purpose of this work was to study the dynamics of structural manifestations of acute cases of postpartum endometritis in cows.
MATERIALS AND METHODS
The light and electron microscopy methods were used when studying structural changes in the endometrium in case of postpartum endometritis in seven cows. Sections of endometrial specimens for light microscopy, 5-7 µm thick, were stained with hematoxylin and eosin and also by Van Gieson's. For electron microscopy, semi-thin sections were stained with Azur-2 in combination with basic fuchsin, as well as contrasting by lead citrate and uranyl acetate.
RESULTS
As a result of the study, we have established the following: Necrobiosis of the epithelial layer of the mucosa, cellular infiltration with shaped elements of blood in the functional layer, swelling of the cells of the uterine gland, and single microbial cells on the surface of the mucosa. We have noted edema of the stroma of the functional layer of the endometrium, swelling of the epithelial layer of the endometrial mucosa, and swelling of fibroblastic and lymphoid cells. Ultrastructural changes in endometrial cells in case of acute postpartum endometritis in cows are accompanied by the destruction of microvilli on the apical surface of the epithelium, an abundance of coccal microflora on the surface of the epithelium, necrobiosis of epithelial cells, and partial edema of the nucleus, and cytoplasm of the histiocyte.
CONCLUSION
We had established that acute purulent-catarrhal dystrophic processes were observed in the structural organization of the endometrium. In the depth of catarrhal mucus on the surface of the endometrium, there was an abundance of bacterial flora, with diplococci being prevalent. In ultrastructural organization of the endometrium, we observed deep dystrophic and necrobiotic processes in the parenchyma and endometrial stroma, as well as exudative processes with a change in the integrity of the microcirculatory bed. Thus, to prevent an inflammatory process from turning into a latent form, it is necessary to detect acute postpartum endometritis promptly using diagnostic methods taking into account the obtained parameters of the dynamics of structural changes in the uterine tissues.
PubMed: 30532504
DOI: 10.14202/vetworld.2018.1473-1478 -
The Journal of Clinical Pediatric... Aug 2020To evaluate the effect of different enamel conditioning protocols and their re-application on the microleakage of fissure sealants placed following saliva contamination.
PURPOSE
To evaluate the effect of different enamel conditioning protocols and their re-application on the microleakage of fissure sealants placed following saliva contamination.
STUDY DESIGN
The study included 156 human third molars in 16 subgroups (2×4×2) under two main groups (sealant type): Group A- hydrophobic resin sealant, 3M Clinpro™ Sealant; Group B- hydrophilic resin sealant, Ultraseal XT Hydro. Each group was then divided according to the type of surface conditioning; 1- Er,Cr:YSSG laser etching, 2- acid-etching, 3- acid-etching+etch-and-rinse adhesive (Prime&Bond® One Select) and 4- self-etching adhesive (Clearfil™ SE Bond). After contaminating the conditioned occlusal enamel surfaces with artificial saliva, fissure sealant was applied in half of the specimens (a), whereas in the other half, (b) the respective surface conditioning was repeated and then fissure sealant was placed. Following thermocycling, the samples were immersed in basic fuchsin, sectioned, and dye penetration was quantitatively assessed with ImageJ. Two-way ANOVA and Bonferroni post-hoc tests were used for statistical analyses (p<0.05).
RESULTS
The least microleakage was observed in A3b and A3a, whereas B4b and B4a were the subgroups with the highest microleakage. Following saliva contamination, when surface conditioning was not re-applied, the effects of fissure sealant types and surface conditioning were significant (p=0.005 and p<0.001, respectively). However, their interaction was insignificant (p=0.173). When surface conditioning was re-applied after saliva contamination, the effects of type of fissure sealant and surface conditioning (p=0.000, for both) and their interaction (p=0.004) were significant.
CONCLUSIONS
3M Clinpro™ Sealant was superior to Ultraseal XT Hydro. Re-application of Er,Cr:YSSG laser and the self-etching adhesive did not affect the microleakage of both fissure sealants. Without re-application, acid-etching+etch-and-rinse adhesive was superior to acid-etching only. However, both of them were similarly successful when they were re-applied following saliva contamination.
Topics: Acid Etching, Dental; Dental Bonding; Dental Enamel; Dental Leakage; Humans; Materials Testing; Pit and Fissure Sealants; Saliva
PubMed: 33167017
DOI: 10.17796/1053-4625-44.4.5 -
International Journal of Biomaterials 2022In recent years, dental composite resins such as tooth-colored restoration are frequently used to restore dental cavities, coronal fractures, and congenital defects....
OBJECTIVE
In recent years, dental composite resins such as tooth-colored restoration are frequently used to restore dental cavities, coronal fractures, and congenital defects. This study aimed to evaluate the microleakage of two methacrylate-based composites (GC Kalore and Luna SDI) in class II restorations.
MATERIALS AND METHODS
In this experimental study, a total of 18 intact human premolars previously extracted for periodontal and orthodontic reasons were randomly divided into two groups. Similar class II cavities (box only) were prepared on all teeth and restored with two different composites. In group 1, a bonding agent (Single Bond 2-SB2; 3M ESPE) and Luna SDI composite in mesial cavities and GC Kalore composite in distal cavities were used. In group 2, Single Bond 2 and GC Kalore composite in mesial cavities and Luna SDI composite in distal cavities were applied. They were then subjected to 2000 thermal cycles in a water bath between 5-55°C (dwell time: 30 seconds in every bath and transfer time: 10 seconds). Then, they were immersed in a 2% basic fuchsin dye solution for 24 hours. After rinsing with water, they were sectioned mesiodistally and evaluated for microleakage using a stereomicroscope.
RESULTS
Independent -test (Mann-Whitney test) showed no statistically significant difference for microleakage in mesial and distal class II restorations between GC Kalore composite and Luna SDI composite ( = 1.000) (= 0.852). A total of 83.4% of the Luna SDI composite samples and 66.6% of the GC Kalore composite had a microleakage score of ≤3 in class II cavities.
CONCLUSION
In the present study, marginal microleakage was found mainly at the gingival floor extending to 1/3 of the axial wall for the Luna SDI composite and GC Kalore composite. Furthermore, no statistically significant difference was found between the microleakage of the Class II cavities restored with Luna SDI composite and GC Kalore composite.
PubMed: 36619594
DOI: 10.1155/2022/3835694 -
Dento Maxillo Facial Radiology May 2022The aim of the present study was to evaluate the effects of 1.5 T and 3 T MRI on the adhesion between the orthodontic brackets and the teeth by evaluating the...
OBJECTIVES
The aim of the present study was to evaluate the effects of 1.5 T and 3 T MRI on the adhesion between the orthodontic brackets and the teeth by evaluating the microleakage between the enamel, adhesive and brackets interfaces.
METHODS
58 extracted human premolars which were received a standard bracket bonding procedure were randomly divided into three groups; control group ( = 20; no MRI), 1.5 T MRI group ( = 19; 20 min MRI exposure of 1.5 T) and 3 T MRI group ( = 19; 20 min MRI exposure of 3 T). The teeth were kept in distiled water for 2 weeks, and thereafter subjected to 500 thermal cycles. Then, specimens were sealed with nail varnish, stained with 0.5% basic fuchsin for 24 h, sectioned and photographed under a stereomicroscope. Microleakage was scored with regard to the adhesive-enamel and bracket-adhesive interfaces at the occlusal and gingival levels. Statistical analysis was accomplished by Kruskal-Wallis and Bonferroni-Dunn tests.
RESULTS
All of the groups exhibited statistically similar microleakage scores in the adhesive-enamel interface along occlusal margins (>0.05, = 0.331). The mean microleakage scores along gingival margins in the 3 T MRI group was significantly higher compared to the control group both in the adhesive-enamel and bracket-adhesive interfaces (<0.05, = 0.019 and = 0.020 respectively). The microleakage scores along the gingival margins were also significantly higher than the occlusal margins in the 3 T MRI group (<0.05, = 0.029).
CONCLUSIONS
3 T MRI may weaken the adhesion between the enamel and the stainless steel orthodontic brackets.
Topics: Composite Resins; Dental Bonding; Dental Leakage; Humans; Magnetic Resonance Imaging; Materials Testing; Orthodontic Brackets; Resin Cements
PubMed: 35113678
DOI: 10.1259/dmfr.20210512 -
Anatomical Record (Hoboken, N.J. : 2007) May 2021Bone is continuously remodeled to repair and strengthen degenerative bone with accumulating dead osteocytes and microfractures. Inner ear osteoprotegerin (OPG)-mediated...
Bone is continuously remodeled to repair and strengthen degenerative bone with accumulating dead osteocytes and microfractures. Inner ear osteoprotegerin (OPG)-mediated inhibition of otic capsular bone remodeling causes excessive perilabyrinthine bone degeneration. Consequently, microcracks accumulate around the inner ear. Microcracks cause osteocyte apoptosis and may disrupt the canalicular network connecting osteocytes. Despite their linear microscopic appearance, microcracks are three-dimensional disruption planes and represent surface areas inside a tissue space. With an elevated microcrack burden the number of disconnected osteocytes is expected to increase. This may prove relevant to ongoing research in otic focal pathologies like otosclerosis. Therefore, an unbiased quantification of the microcrack surface density (mm /mm ) in the human otic capsule is essential. In this study unbiased stereology was applied to undecalcified bulk stained human temporal bones to demonstrate its feasibility in describing the three-dimensional reality behind two dimensional observations of microcracks. A total of 28 human temporal bones and five ribs were bulk stained in basic fuchsin, serially sectioned and hand-ground to a thickness of 80-120 μm. Both horizontal and vertical sections were produced and compared. This study showed that surface density of microcracks was significantly higher around the inner ear compared to ribs. Furthermore, no significant difference in microcrack surface density between horizontal and vertical sections in the temporal bone was demonstrated.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Bone Remodeling; Child; Child, Preschool; Ear, Inner; Female; Humans; Infant; Infant, Newborn; Male; Middle Aged; Osteocytes; Otosclerosis; Ribs; Temporal Bone; Young Adult
PubMed: 33040475
DOI: 10.1002/ar.24535